Roderick Cassar, 41, was arrested early on Wednesday in a raid on his residence in Qrendi following a standoff which lasted 17 hours.
Armed officers wearing protective gear entered his house at 0230am, firing a number of flash blanks. Cassar was arrested unharmed but was given a medical checkup on the scene.
The gun allegedly used in the murder of his wife, Bernice, on Tuesday in Corradino was also recovered. This followed several hours of negotiations urging Cassar to turn himself in.
Cassar is expected to undergo interrogation on Wednesday, with the police having 48 hours from the time they arrest a suspect to charge or release him.
Bernice Cassar, the mother of two children with Roderick, was shot twice on a road behind the Paola MCAST campus as she was driving to work. Her body was fund outside her Nissan Qashqai.
It was revealed that Bernice had filed several domestic violence reports against her husband, which led to the police to press criminal charges against him last May. Roderick continuously violated court order demanding he does not approach his wife.